Construction and characterization of peroxisome proliferator-activated receptor-gamma co-activator 1 alpha (PGC-1 alpha over-expressing cell line derived from human hepatocyte carcinoma HepG2 cells)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F13%3A10146004" target="_blank" >RIV/00216208:11160/13:10146004 - isvavai.cz</a>

Result on the web

<a href="http://biomed.papers.upol.cz/pdfs/bio/2013/03/02.pdf" target="_blank" >http://biomed.papers.upol.cz/pdfs/bio/2013/03/02.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5507/bp.2012.075" target="_blank" >10.5507/bp.2012.075</a>

Result language

angličtina

Original language name

Construction and characterization of peroxisome proliferator-activated receptor-gamma co-activator 1 alpha (PGC-1 alpha over-expressing cell line derived from human hepatocyte carcinoma HepG2 cells)

Original language description

Aims. The aim was develop stable human cell line stable over-expressing transcription co-activator peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC-1 alpha) with restored hepatospecific functions and increased expression of major xenobiotic metabolizing enzymes. Methods. Six clones of HepG2-PGC-1 alpha and one control clone HepG2-pcDNA3 were isolated and analyzed for secretion of hepatospecific markers, fibrinogen, albumin and alpha1-antitrypsin. Expression levels of protein and mRNA of hepatocyte nuclear factor (HNF4 alpha), pregnane X receptor (PXR) and aryl hydrocarbon receptor (AhR) were determined. We measured basal and ligand inducible expression of CYP1A1 and CYP3A4. Results. Stably transfected cell line HepG2-PGC-1 alpha derived from HepG2 cells over-expressing PGC-1 alpha displayed increased secretion of fibrinogen, but not albumin or alpha1-antitrypsin compared to parent HepG2 cells. We found increased levels of HNF4 alpha, PXR and AhR proteins but n

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FR - Pharmacology and apothecary chemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia

ISSN

1213-8118

e-ISSN

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Volume of the periodical

157

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

214-221

UT code for WoS article

000329091100003

EID of the result in the Scopus database

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