Construction and characterization of peroxisome proliferator-activated receptor-gamma co-activator 1 alpha (PGC-1alfa over-expressing cell line derived from human hepatocyte carcinoma HepG2 cells)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33147426" target="_blank" >RIV/61989592:15310/13:33147426 - isvavai.cz</a>
Result on the web
<a href="http://biomed.papers.upol.cz/pdfs/bio/2013/03/02.pdf" target="_blank" >http://biomed.papers.upol.cz/pdfs/bio/2013/03/02.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5507/bp.2012.075" target="_blank" >10.5507/bp.2012.075</a>

Result language
angličtina
Original language name
Construction and characterization of peroxisome proliferator-activated receptor-gamma co-activator 1 alpha (PGC-1alfa over-expressing cell line derived from human hepatocyte carcinoma HepG2 cells)
Original language description
AIMS: The aim was develop stable human cell line stable over-expressing transcription co-activator peroxisome proliferator-activated receptor gamma co-activator 1alfa (PGC-1alfa) with restored hepatospecific functions and increased expression of major xenobiotic metabolizing enzymes. METHODS: Six clones of HepG2-PGC-1alfa and one control clone HepG2-pcDNA3 were isolated and analyzed for secretion of hepatospecific markers, fibrinogen, albumin and alpha1-antitrypsin. Expression levels of protein and mRNAof hepatocyte nuclear factor (HNF4alfa), pregnane X receptor (PXR) and aryl hydrocarbon receptor (AhR) were determined. We measured basal and ligand inducible expression of CYP1A1 and CYP3A4. RESULTS: Stably transfected cell line HepG2-PGC-1alfa derivedfrom HepG2 cells over-expressing PGC-1alfa displayed increased secretion of fibrinogen, but not albumin or alpha1-antitrypsin compared to parent HepG2 cells. We found increased levels of HNF4alfa, PXR and AhR proteins but not their mRNAs
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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