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Francisella tularensis subsp holarctica DsbA homologue: a thioredoxin-like protein with chaperone function

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F13%3A10210411" target="_blank" >RIV/00216208:11160/13:10210411 - isvavai.cz</a>

  • Alternative codes found

    RIV/60162694:G44__/13:43874878

  • Result on the web

    <a href="http://mic.sgmjournals.org/content/159/Pt_11/2364.full" target="_blank" >http://mic.sgmjournals.org/content/159/Pt_11/2364.full</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1099/mic.0.070516-0" target="_blank" >10.1099/mic.0.070516-0</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Francisella tularensis subsp holarctica DsbA homologue: a thioredoxin-like protein with chaperone function

  • Original language description

    Francisella tularensis is a highly infectious facultative intracellular bacterium and aetiological agent of tularaemia. The conserved hypothetical lipoprotein with homology to thiol/disulphide oxidoreductase proteins (FtDsbA) is an essential virulence factor in F. tularensis. Its protein sequence has two different domains: the DsbA_Com1_like domain (DSBA), with the highly conserved catalytically active site CXXC and cis-proline residue; and the domain amino-terminal to FKBP-type peptidyl-prolyl isomerases (FKBP_N). To establish the role of both domains in tularaemia infection models, site-directed and deletion mutagenesis affecting the active site (AXXA), the cis-proline (P286T) and the FKBP_N domain (Delta FKBP_N) were performed. The generated mutations led to high attenuation with the ability to induce full or partial host protective immunity. Recombinant protein analysis revealed that the active site CXXC as well as the cis-proline residue and the FKBP_N domain are necessary for cor

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2013

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Microbiology

  • ISSN

    1350-0872

  • e-ISSN

  • Volume of the periodical

    159

  • Issue of the periodical within the volume

    November

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    11

  • Pages from-to

    2364-2374

  • UT code for WoS article

    000328517600015

  • EID of the result in the Scopus database