Cooperation of both, the FKBP N-like and the DSBA-like, domains is necessary for the correct function of FTS 1067 protein involved in Francisella tularensis virulence and pathogenesis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F15%3A43875427" target="_blank" >RIV/60162694:G44__/15:43875427 - isvavai.cz</a>

Result on the web

<a href="http://femspd.oxfordjournals.org/content/73/6/ftv030" target="_blank" >http://femspd.oxfordjournals.org/content/73/6/ftv030</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/femspd/ftv030" target="_blank" >10.1093/femspd/ftv030</a>

Result language

angličtina

Original language name

Cooperation of both, the FKBP N-like and the DSBA-like, domains is necessary for the correct function of FTS 1067 protein involved in Francisella tularensis virulence and pathogenesis

Original language description

Francisella tularensis the etiological agent of tularaemia is one of the most infectious human pathogen known. Our knowledge about its key virulence factors has increased recently but it still remains a lot to explore. One of the described essential virulence factors is membrane lipoprotein FTS 1067 (nomenclature of F. tularensis subsp. holarctica strain FSC200) with homology to the protein family of disulphide oxidoreductases DsbA. Lipoprotein consists of two different domains: the C-terminal DsbA Com1-like domain (DSBA-like) and the N-terminal FKBP-type peptidyl-prolyl cis/trans isomerases (FKBP N-like). To uncover the biological role of these domains, we created bacterial strain with deletion of the DSBA-like domain. This defect in gene coding for lipoprotein FTS 1067 led to high in vivo attenuation associated with the ability to induce host protective immunity. Analyses performed with the truncated recombinant protein showed that the absence of DSBA-like domain revealed the loss of

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Pathogens and Disease

ISSN

2049-632X

e-ISSN

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Volume of the periodical

73

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

"Article Number: UNSP ftv030"

UT code for WoS article

000362574300002

EID of the result in the Scopus database

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