Identification of two substrates of FTS_ 1067 protein – an essential virulence factor of Francisella tularensis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F17%3A43875741" target="_blank" >RIV/60162694:G44__/17:43875741 - isvavai.cz</a>
Result on the web
<a href="http://akademiai.com/doi/abs/10.1556/030.63.2016.013" target="_blank" >http://akademiai.com/doi/abs/10.1556/030.63.2016.013</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1556/030.63.2016.013" target="_blank" >10.1556/030.63.2016.013</a>
Alternative languages
Result language
angličtina
Original language name
Identification of two substrates of FTS_ 1067 protein – an essential virulence factor of Francisella tularensis
Original language description
Francisella tularensis is a highly virulent intracellular pathogen with the capacity to infect a variety of hosts including humans. One of the most important proteins involved in F. tularensis virulence and pathogenesis is the protein DsbA. This protein is annotated as a lipoprotein with disulfide oxidoreductase/isomerase activity. Therefore, its interactions with different substrates, including probable virulence factors, to assist in their proper folding are anticipated. We aimed to use the immunopurification approach to find DsbA (gene locus FTS_1067) interacting partners in F. tularensis subsp. holarctica strain FSC200 and compare the identified substrates with proteins which were found in our previous comparative proteome analysis. As a result of our work two FTS_1067 substrates, D-alanyl-D-alanine carboxypeptidase family protein and HlyD family secretion protein, were identified. Bacterial two-hybrid systems were further used to test their relevance in confirming FTS_1067 protein interactions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Microbiologica et Immunologica Hungarica
ISSN
1217-8950
e-ISSN
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Volume of the periodical
64
Issue of the periodical within the volume
1
Country of publishing house
HU - HUNGARY
Number of pages
13
Pages from-to
37-49
UT code for WoS article
000398838400004
EID of the result in the Scopus database
2-s2.0-85016980207