Francisella tularensis type B Delta dsbA mutant protects against type A strain and induces strong inflammatory cytokine and Th1-like antibody response in vivo

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F15%3A00506543" target="_blank" >RIV/61388971:_____/15:00506543 - isvavai.cz</a>

Alternative codes found

RIV/60162694:G44__/15:43875429

Result on the web

<a href="https://academic.oup.com/femspd/article/73/8/ftv058/2467550" target="_blank" >https://academic.oup.com/femspd/article/73/8/ftv058/2467550</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/femspd/ftv058" target="_blank" >10.1093/femspd/ftv058</a>

Result language

angličtina

Original language name

Francisella tularensis type B Delta dsbA mutant protects against type A strain and induces strong inflammatory cytokine and Th1-like antibody response in vivo

Original language description

Francisella tularensis subspecies tularensis is a highly virulent intracellular bacterial pathogen, causing the disease tularemia. However, a safe and effective vaccine for routine application against F. tularensis has not yet been developed. We have recently constructed the deletion mutants for the DsbA homolog protein (Delta dsbA/FSC200) and a hypothetical protein IglH (Delta iglH/FSC200) in the type B F. tularensis subsp. holarctica FSC200 strain, which exerted different protection capacity against parental virulent strain. In this study, we further investigated the immunological correlates for these different levels of protection provided by Delta dsbA/FSC200 and Delta iglH/FSC200 mutants. Our results show that Delta dsbA/FSC200 mutant, but not Delta iglH/FSC200 mutant, induces an early innate inflammatory response leading to strong Th1-like antibody response. Furthermore, vaccination with Delta dsbA/FSC200 mutant, but not with Delta iglH/FSC200, elicited protection against the subsequent challenge with type A SCHU S4 strain in mice. An immunoproteomic approach was used to map a spectrum of antigens targeted by Th1-like specific antibodies, and more than 80 bacterial antigens, including novel ones, were identified. Comparison of tularemic antigens recognized by the Delta dsbA/FSC200 post-vaccination and the SCHU S4 post-challenge sera then revealed the existence of 22 novel SCHU S4 specific antibody clones.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Pathogens and Disease

ISSN

2049-632X

e-ISSN

—

Volume of the periodical

73

Issue of the periodical within the volume

8

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

ftv058

UT code for WoS article

000362978200003

EID of the result in the Scopus database

2-s2.0-85010004132