Micellar electrokinetic chromatography with short-end injection for rapid separation and simultaneous determination of aesculin, aesculetin, and phenylephrine in pharmaceutical preparations
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F15%3A10317293" target="_blank" >RIV/00216208:11160/15:10317293 - isvavai.cz</a>
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1002/elps.201500215/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/elps.201500215/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201500215" target="_blank" >10.1002/elps.201500215</a>
Alternative languages
Result language
angličtina
Original language name
Micellar electrokinetic chromatography with short-end injection for rapid separation and simultaneous determination of aesculin, aesculetin, and phenylephrine in pharmaceutical preparations
Original language description
A fast micellar electrokinetic chromatography (MEKC) method for simultaneous assay of aesculin, aesculetin, and phenylephrine was developed and validated. The separation was carried out in a fused-silica capillary (50 mu m id, total length 64.5 cm, effective length 8.5 cm) with UV detection at 210 nm, temperature 25 degrees C and separation voltage -25 kV. The samples were loaded hydrodynamically at a pressure of -50 mbar for 6 s. The background electrolyte of pH 8.6 contained 20 mM boric acid, 60 mM SDS, and 5% (v/v) of methanol. The calibration curves were linear in the range 10-500 mu g/mL for aesculin and aesculetin and 12.5-625 mu g/mL for phenylephrine. The RSD values of corrected peak areas were 0.6-1.2% (n = 6) when determining 0.2 mg/mL of aesculin and aesculetin and 0.25 mg/mL of phenylephrine in prepared standard mixtures. The method was successfully applied to the assay of aesculin and phenylephrine in a pharmaceutical preparation (RSD = 1.9-2.0%; n = 3) and the robustness
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/EE2.3.30.0061" target="_blank" >EE2.3.30.0061: Increasing of the R&D capacity at Charles University through new positions for graduates of doctoral studies</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
36
Issue of the periodical within the volume
21
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
2754-2761
UT code for WoS article
000365620500015
EID of the result in the Scopus database
2-s2.0-84947866754