A new approach to the rapid separation of isomeric compounds in a Silybum marianum extract using UHPLC core-shell column with F5 stationary phase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10362178" target="_blank" >RIV/00216208:11160/17:10362178 - isvavai.cz</a>

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S0731708516304885" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0731708516304885</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jpba.2016.11.042" target="_blank" >10.1016/j.jpba.2016.11.042</a>

Result language

angličtina

Original language name

A new approach to the rapid separation of isomeric compounds in a Silybum marianum extract using UHPLC core-shell column with F5 stationary phase

Original language description

In this paper, a new ultra-high performance liquid chromatography (UHPLC) method using a core-shell column with a pentafluorophenyl stationary phase for separation of seven active compounds of a Silybum marianum extract was developed and validated. Silymarin, an extract of Silybum marianum, is known for its abilities to protect the liver from toxic substances, hepatitis therapy, and anti-tumour activity. Silymarin is currently being widely used in commercial preparations and herbal teas. Separation of seven compounds contained in the Silybum marianum extract (taxifolin, silychristin, silydianin, silybin A, silybin B, isosilybin A, isosilybin B) and other substances occurring in real samples was performed on the Kinetex 1.7 mu F5 100A (150 x 2.1 mm), 1.7 mu m particle size core-shell column, with a mobile phase methanol/100 mM phosphate buffer pH 2.0 according to the gradient program. A mobile phase 0.35 mL min(-1) flow rate and 50 degrees C temperature was used for the separation. The detection wavelength was set at 288 nm. Under optimal chromatographic conditions, good linearity with a correlation coefficient of R(2) &gt; 0.999 for all compounds was achieved. The available commercial samples of herbal teas and food supplements were extracted with methanol using an ultrasonic bath. After dilution with water and centrifugation, a 2 mu L sample of the filtered supernatant was directly injected into the UHPLC system. The use of a pentafluorophenyl stationary phase with methanol as the organic component of the mobile phase showed new ways to effectively separate isomeric compounds in herbal extracts, which could not be done with the conventional C18 stationary phase.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30104 - Pharmacology and pharmacy

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Pharmaceutical and Biomedical Analysis

ISSN

0731-7085

e-ISSN

—

Volume of the periodical

134

Issue of the periodical within the volume

February

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

203-213

UT code for WoS article

000392909900026

EID of the result in the Scopus database

2-s2.0-85002374694