Stability Indicating Method for Determination of Sodium Picosulfate in Pharmaceutical Preparation - Comparison of HPLC, UHPLC and HTLC
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10364425" target="_blank" >RIV/00216208:11160/17:10364425 - isvavai.cz</a>
Result on the web
<a href="http://www.eurekaselect.com/142317/article" target="_blank" >http://www.eurekaselect.com/142317/article</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2174/1573412912666160518145632" target="_blank" >10.2174/1573412912666160518145632</a>
Alternative languages
Result language
angličtina
Original language name
Stability Indicating Method for Determination of Sodium Picosulfate in Pharmaceutical Preparation - Comparison of HPLC, UHPLC and HTLC
Original language description
Background: Various chromatographic modes were used for the evaluation of liquid pharmaceutical preparation containing favorite laxative drug sodium picosulfate. Objective: The aim of this study was to compare capabilities of conventional HPLC, UHPLC and HTLC on the example of stability indicating methods with UV 263 nm detection for simultaneous determination of sodium picosulfate, its two degradation products and sodium benzoate in oral liquid pharmaceutical preparation. Method: A novel money-saving preparation of system suitability test solution using partial acid hydrolysis yielding main degradation products was developed. Developed HPLC system with RP-C18e column used acetonitrile - propan-2-ol - phosphate puffer pH 7.0 with addition of cetyltrimethylammonium bromide (43: 2: 55, v/v/v) as mobile phase, 1 ml/min, column temperature 40 degrees C. UHPLC separation was carried out using core-shell RP-C18 column under the same chromatographic conditions, flow-rate 0.5 ml/min. HTLC method used the same column as in UHPLC, mobile phase acetonitrile -30 mM triethylamine phosphate buffer pH 5.0 (10: 90, v/v), flow-rate 0.5 ml/min and column temperature 100 degrees C. Results: HPLC method was very reliable and robust but rather time consuming, whereas UHPLC brought 5times faster analysis with 10times lower solvent consumption. High temperature during HTLC separation did not affect the stability of the tested compounds, and organic solvents consumption was 8times lower. Conclusion: Although all developed methods were satisfactorily validated, UHPLC is the most suitable technique for routine analysis or stability studies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30104 - Pharmacology and pharmacy
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Current Pharmaceutical Analysis
ISSN
1573-4129
e-ISSN
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Volume of the periodical
13
Issue of the periodical within the volume
3
Country of publishing house
AE - UNITED ARAB EMIRATES
Number of pages
6
Pages from-to
250-255
UT code for WoS article
000402460300007
EID of the result in the Scopus database
2-s2.0-85019749394