Determination of the purity of phenoxymethylpenicillin by micellar electrokinetic chromatography and reversed phase liquid chromatography on a monolithic silica column

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023817%3A_____%2F03%3A%230000001" target="_blank" >RIV/00023817:_____/03:#0000001 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/jssc.200301441" target="_blank" >http://dx.doi.org/10.1002/jssc.200301441</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.200301441" target="_blank" >10.1002/jssc.200301441</a>

Result language

angličtina

Original language name

Determination of the purity of phenoxymethylpenicillin by micellar electrokinetic chromatography and reversed phase liquid chromatography on a monolithic silica column

Original language description

A micellar electrokinetic chromatographic and a fast reversed-phase liquid chromatographic method have been developed for determination of the purity of phenoxymethylpenicillin. The optimized running buffer composition was 40 mM phosphate?borate?125 mM SDS?3.5% (v/v) methanol. The HPLC method employed a monolithic silica C18 column and a mobile phase composed of phosphate buffer, pH 3.5, and ACN, the flow-rate being 3.5 mL/min. Both methods were successfully validated. Linearity, intermediate precision,limits of quantitation, accuracy, and a good correlation of the HPLC and MEKC results were demonstrated. Both methods proved to be fast and reliable and sufficiently sensitive. A combination of the two methods can be very useful in impurity profiling.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/NL5965" target="_blank" >NL5965: Quality control of amoxicillin,ampicillin and phenoxymethylpenicillin in pharmaceutical preparations using HPLC and CE tests for related compounds</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of separation science

ISSN

1615-9314

e-ISSN

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Volume of the periodical

26

Issue of the periodical within the volume

8

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

701-708

UT code for WoS article

000183660100010

EID of the result in the Scopus database

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