Determination of the purity of ampicillin by micellar electrokinetic chromatography and reversed phase liquid chromatography on a monolithic silica column

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023817%3A_____%2F04%3A%230000002" target="_blank" >RIV/00023817:_____/04:#0000002 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/jssc.200301680" target="_blank" >http://dx.doi.org/10.1002/jssc.200301680</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.200301680" target="_blank" >10.1002/jssc.200301680</a>

Result language

angličtina

Original language name

Determination of the purity of ampicillin by micellar electrokinetic chromatography and reversed phase liquid chromatography on a monolithic silica column

Original language description

A micellar electrokinetic chromatographic (MEKC) method and a fast reversed-phase liquid chromatographic one have been developed for determining the purity of ampicillin. MEKC separation of ampicillin and its related substances was performed with the useof an untreated fused-silica capillary and 40 mM phosphate-borate buffer, pH 7.5 containing 75 mM SDS. The HPLC method employed a monolithic silica C18 column and a mobile phase composed of phosphate buffer, pH 5.2 and ACN, the flow rate being 4.0 mL/min. Both methods were successfully validated. Linearity, relative response factors, limits of quantitation, intermediate precision, and accuracy were evaluated. The methods proved to be fast, reliable, and sufficiently sensitive and, accordingly, well-suited for control of purity of ampicillin substance, injections, and capsules. A combination of both methods can be very useful in the confirmation of impurity profiles.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/NL5965" target="_blank" >NL5965: Quality control of amoxicillin,ampicillin and phenoxymethylpenicillin in pharmaceutical preparations using HPLC and CE tests for related compounds</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of separation science

ISSN

1615-9314

e-ISSN

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Volume of the periodical

27

Issue of the periodical within the volume

7-8

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

560-568

UT code for WoS article

000221547900012

EID of the result in the Scopus database

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