Fast separation of red colorants in beverages using cyano monolithic column in Sequential Injection Chromatography

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10364766" target="_blank" >RIV/00216208:11160/17:10364766 - isvavai.cz</a>

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S0026265X16302971" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0026265X16302971</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2016.10.022" target="_blank" >10.1016/j.microc.2016.10.022</a>

Result language

angličtina

Original language name

Fast separation of red colorants in beverages using cyano monolithic column in Sequential Injection Chromatography

Original language description

Flexible medium-pressure Sequential Injection Chromatography (SIC) flow manifold was used for development of a fast green chromatography method. The article presents novel use of a cyano monolithic column (Merck Chromolith 50 mm x 4.6 mm) for a separation of three water soluble colorants (carmoisine, Ponceau 4R and red 2G), aiming the extension of the SIC separation capabilities for polar analytes. The separation was performed in a gradient elution mode with changing concentration (0.05-0.005% v/v) of ammonium phosphate at pH 6.0 as a mobile phase. No organic modifier was used in mobile phase, thus the green chromatography rules were fulfilled. The vis spectrophotometric detection was set at 500 nm. High performance of the separation with the cyano monolithic column was described and discussed (peak capacities 6.67-10.94, peak symmetries 1.11-1.50, resolutions &gt;= 2.78 and time for one analysis 3.15 min). The method was represented by analytical parameters - LODs of 0.45 mg L-1, a LOQs of 1.5 mg L-1 and calibration range of 1.5-60.0 mg L-1 (r &gt; 0.997) for all analytes, and a method repeatability at 10 mg L-1 of RSD &lt;= 2.69% (n = 5) and system precision of RSD &lt; 1.55% (n = 6). The developed method was used for determination of carmoisine and Ponceau 4R in red coloured beverages with red 2G as an internal standard. The results confirmed benefits of extended chromatographic selectivity of cyano monolithic column in the SIC.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30104 - Pharmacology and pharmacy

Project

<a href="/en/project/GA15-10781S" target="_blank" >GA15-10781S: On-line hyphenation of automated extraction processes with liquid chromatography for complete sample analysis</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microchemical Journal

ISSN

0026-265X

e-ISSN

—

Volume of the periodical

130

Issue of the periodical within the volume

January

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

384-389

UT code for WoS article

000390072400054

EID of the result in the Scopus database

2-s2.0-84994017856