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Phase separation in ceramide[NP] containing lipid model membranes: neutron diffraction and solid-state NMR

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10364881" target="_blank" >RIV/00216208:11160/17:10364881 - isvavai.cz</a>

  • Result on the web

    <a href="http://pubs.rsc.org/en/content/articlehtml/2017/sm/c6sm02356h" target="_blank" >http://pubs.rsc.org/en/content/articlehtml/2017/sm/c6sm02356h</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1039/c6sm02356h" target="_blank" >10.1039/c6sm02356h</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Phase separation in ceramide[NP] containing lipid model membranes: neutron diffraction and solid-state NMR

  • Original language description

    The stratum corneum is the outermost layer of the skin and protects the organism against external influences as well as water loss. It consists of corneocytes embedded in a mixture of ceramides, fatty acids, and cholesterol in a molar ratio of roughly 1 : 1 : 1. The unique structural and compositional arrangement of these stratum corneum lipids is responsible for the skin barrier properties. Many studies investigated the organization of these barrier lipids and, in particular, the exact conformation of ceramides. However, so far no consensus has been reached. In this study, we investigate a model system comprised of N-(nonhydroxy-tetracosanoyl)-phytosphingosine/cholesterol/tetracosanoic acid (CER[NP]-C24/CHOL/TA) at a 1 : 1 : 1 molar ratio using neutron diffraction and H-2 solid-state NMR spectroscopy at temperatures from 25 degrees C to 80 degrees C. Deuterated variants of all three lipid components of the model system were used to enable their separate investigation in the NMR spectra and quantification of the amount of molecules in each phase. Neutron scattering experiments show the coexistence of two lipid phases at low temperatures with repeat spacings of 54.2 angstrom and 43.0 angstrom at a physiological skin temperature of 32 degrees C. They appear to be indistinguishable in the H-2 NMR spectra as both phases are crystalline and ceramide molecules do not rotate around their long axis on a microsecond timescale. The evolution of these phases upon heating is followed and with increasing temperature fluid and even isotropically mobile molecules are observed. A model of the organization of the lamellar phases is proposed in which the thicker phase consists of CER[NP]-C24 in a hairpin conformation mixed with CHOL and TA, while the phase with a repeat spacing of 43.0 angstrom contains CER[NP]-C24 in a V-shape conformation.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30104 - Pharmacology and pharmacy

Result continuities

  • Project

    <a href="/en/project/GA13-23891S" target="_blank" >GA13-23891S: Lipid membrane models – a new tool for studying pathophysiology of skin diseases at a molecular level</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Soft Matter

  • ISSN

    1744-683X

  • e-ISSN

  • Volume of the periodical

    13

  • Issue of the periodical within the volume

    10

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    13

  • Pages from-to

    2107-2119

  • UT code for WoS article

    000396291300018

  • EID of the result in the Scopus database

    2-s2.0-85015018363