Probing the role of ceramide hydroxylation in skin barrier lipid models by H-2 solid-state NMR spectroscopy and X-ray powder diffraction

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F18%3A10382595" target="_blank" >RIV/00216208:11160/18:10382595 - isvavai.cz</a>

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S0005273618300440" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0005273618300440</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbamem.2018.02.003" target="_blank" >10.1016/j.bbamem.2018.02.003</a>

Result language

angličtina

Original language name

Probing the role of ceramide hydroxylation in skin barrier lipid models by H-2 solid-state NMR spectroscopy and X-ray powder diffraction

Original language description

this work, we studied model stratum corneum lipid mixtures composed of the hydroxylated skin ceramides Nlignoceroyl 6-hydroxysphingosine (Cer[NH]) and alpha-hydroxylignoceroyl phytosphingosine (Cer[AP]). Two model skin lipid mixtures of the composition Cer[NH] or Cer[AP], N-lignoceroyl sphingosine (Cer[NS]), lignoceric acid (C24:0) and cholesterol in a 0.5:0.5:1:1 molar ratio were compared. Model membranes were investigated by differential scanning calorimetry and H-2 solid-state NMR spectroscopy at temperatures from 25 degrees C to 80 degrees C. Each component of the model mixture was specifically deuterated for selective detection by 2H NMR. Thus, the exact phase composition of the mixture at varying temperatures could be quantified. Moreover, using X-ray powder diffraction we investigated the lamellar phase formation. From the solid-state NMR and DSC studies, we found that both hydroxylated Cer[NH] and Cer[AP] exhibit a similar phase behavior. At physiological skin temperature of 32 degrees C, the lipids form a crystalline (orthorhombic) phase. With increasing temperature, most of the lipids become fluid and form a liquid-crystalline phase, which converts to the isotropic phase at higher temperatures (65-80 degrees C). Interestingly, lignoceric acid in the Cer[NH]-containing mixture has a tendency to form two types of fluid phases at 65 degrees C. This tendency was also observed in Cer[AP]-containing membranes at 80 degrees C. While Cer[AP]-containing lipid models formed a short periodicity phase featuring a repeat spacing of d = 5.4 nm, in the Cer[NH]-based model skin lipid membranes, the formation of unusual long periodicity phase with a repeat spacing of d = 10.7 nm was observed.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30104 - Pharmacology and pharmacy

Project

<a href="/en/project/GC16-25687J" target="_blank" >GC16-25687J: Interdependencies between Inflammatory Processes and Barrier Lipids in Diseased Skin</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochimica et Biophysica Acta - Biomembranes

ISSN

0005-2736

e-ISSN

—

Volume of the periodical

1860

Issue of the periodical within the volume

5

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

1162-1170

UT code for WoS article

000435057700024

EID of the result in the Scopus database

2-s2.0-85042066222