Degradation of ouabain in 80-year-old injection solution studied by HILIC-MS

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F18%3A10381599" target="_blank" >RIV/00216208:11160/18:10381599 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/18:10381599
Result on the web
<a href="http://link.springer.com/article/10.1007/s00706-018-2216-9" target="_blank" >http://link.springer.com/article/10.1007/s00706-018-2216-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00706-018-2216-9" target="_blank" >10.1007/s00706-018-2216-9</a>

Result language
angličtina
Original language name
Degradation of ouabain in 80-year-old injection solution studied by HILIC-MS
Original language description
An 80-year-old injection solution of ouabain was analyzed using HPLC-MS. A new separation method involving hydrophilic interaction liquid chromatography (HILIC) using an amide-based stationary phase with mass spectrometric detection was developed for the analysis. The optimized mobile phase composition was acetonitrile:water 85:15 (v/v) at the flow rate of 0.2 cm(3)/min under the isocratic elution. The ouabain content was found reduced to 56% of declared content and probable degradation product with oxidized hydroxy group was established. The ESI-MS2 fragmentation mechanism of ouabain was proposed.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry

Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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