Induction of xenobiotic-metabolizing enzymes in hepatocytes by beta-naphthoflavone: Time-dependent changes in activities, protein and mRNA levels

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F18%3A10382497" target="_blank" >RIV/00216208:11160/18:10382497 - isvavai.cz</a>

Result on the web

<a href="http://content.sciendo.com/view/journals/acph/68/1/article-p75.xml" target="_blank" >http://content.sciendo.com/view/journals/acph/68/1/article-p75.xml</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/acph-2018-0005" target="_blank" >10.2478/acph-2018-0005</a>

Result language

angličtina

Original language name

Induction of xenobiotic-metabolizing enzymes in hepatocytes by beta-naphthoflavone: Time-dependent changes in activities, protein and mRNA levels

Original language description

In the present study, time-dependency of the induction effect of a selective inducer on the activity, protein and mRNA levels of cytochromes P450 1A1/2 (CYP1A1/2), NAD(P)H:quinone oxidoreductase 1 (NQO1) and glutathione S-transferases (GSTA), in primary culture of rat hepatocytes was tested and evaluated. To show the differences in responses of tested enzymes, the common aryl hydrocarbon receptor (AhR) ligand agonist, beta-naphthoflavone (BNF), was used. Induction of CYP1A1/2 by BNF was detected at all time intervals and at all levels (i. e., mRNA, protein, enzyme activity). Different responses of NQO1 and GSTA upon BNF treatment were observed. Our results demonstrate that the responses of different xenobiotic-metabolizing enzymes to the inducer vary in time and depend on the measured parameter. For these reasons, an induction study featuring only one-time interval treatment and/or one parameter testing could produce misleading information.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10600 - Biological sciences

Project

<a href="/en/project/GBP303%2F12%2FG163" target="_blank" >GBP303/12/G163: Centre of drug-dietary supplements interactions and nutrigenetics</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Pharmaceutica

ISSN

1330-0075

e-ISSN

—

Volume of the periodical

68

Issue of the periodical within the volume

1

Country of publishing house

HR - CROATIA

Number of pages

11

Pages from-to

75-85

UT code for WoS article

000425292600006

EID of the result in the Scopus database

2-s2.0-85042682585