Ultra-rapid auxin metabolite profiling for high-throughput mutant screening in Arabidopsis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F18%3A10383055" target="_blank" >RIV/00216208:11160/18:10383055 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/18:00489589 RIV/61989592:15310/18:73592226

Result on the web

<a href="http://academic.oup.com/jxb/article/69/10/2569/4919650" target="_blank" >http://academic.oup.com/jxb/article/69/10/2569/4919650</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jxb/ery084" target="_blank" >10.1093/jxb/ery084</a>

Result language

angličtina

Original language name

Ultra-rapid auxin metabolite profiling for high-throughput mutant screening in Arabidopsis

Original language description

Auxin (indole-3-acetic acid, IAA) plays fundamental roles as a signalling molecule during numerous plant growth and development processes. The formation of local auxin gradients and auxin maxima/minima, which is very important for these processes, is regulated by auxin metabolism (biosynthesis, degradation, and conjugation) as well as transport. When studying auxin metabolism pathways it is crucial to combine data obtained from genetic investigations with the identification and quantification of individual metabolites. Thus, to facilitate efforts to elucidate auxin metabolism and its roles in plants, we have developed a high-throughput method for simultaneously quantifying IAA and its key metabolites in minute samples (&lt;10 mg FW) of Arabidopsis thaliana tissues by in-tip micro solid-phase extraction and fast LC-tandem MS. As a proof of concept, we applied the method to a collection of Arabidopsis mutant lines and identified lines with altered IAA metabolite profiles using multivariate data analysis. Finally, we explored the correlation between IAA metabolite profiles and IAA-related phenotypes. The developed rapid analysis of large numbers of samples (&gt;100 samples d(-1)) is a valuable tool to screen for novel regulators of auxin metabolism and homeostasis among large collections of genotypes.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

—

Volume of the periodical

69

Issue of the periodical within the volume

10

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

2569-2579

UT code for WoS article

000431251900011

EID of the result in the Scopus database

2-s2.0-85046280384