Determination of tetracyclines in human urine samples by capillary electrophoresis in combination with field amplified sample injection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F18%3A10393264" target="_blank" >RIV/00216208:11160/18:10393264 - isvavai.cz</a>

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1002/elps.201700288" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/elps.201700288</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201700288" target="_blank" >10.1002/elps.201700288</a>

Result language

angličtina

Original language name

Determination of tetracyclines in human urine samples by capillary electrophoresis in combination with field amplified sample injection

Original language description

A sensitive method using CZE-UV detection has been developed for the determination of five tetracycline antibiotics in human urine samples. To improve the sensitivity of the method, an on-line preconcentration strategy, named field-amplified sample injection, has been developed, based on the electrokinetic injection of the sample, which requires only a 1:100 dilution with sample solvent before injection. Under optimum conditions, sensitivity enhancement factors ranged from 450 to 800 for the studied compounds. The applicability of the proposed method was demonstrated by the determination of these antibiotics in spiked urine samples. The limits of quantification were lower than 0.8 mg/L and the precision (intra- and inter-day), expressed as %RSD was below 14%. Recoveries ranged from 92.1 to 96.7%. Thus, the proposed procedure is a simple, fast and efficient strategy which could be used as therapeutic drug monitoring in human urine samples.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30104 - Pharmacology and pharmacy

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

—

Volume of the periodical

39

Issue of the periodical within the volume

4

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

608-615

UT code for WoS article

000425018200005

EID of the result in the Scopus database

2-s2.0-85036499418