DNA-DOPE-gemini surfactants complexes at low surface charge density: from structure to transfection efficiency

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F18%3A10393269" target="_blank" >RIV/00216208:11160/18:10393269 - isvavai.cz</a>

Result on the web

<a href="http://www.elis.sk/download_file.php?product_id=5546" target="_blank" >http://www.elis.sk/download_file.php?product_id=5546</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4149/gpb_2017042" target="_blank" >10.4149/gpb_2017042</a>

Result language

angličtina

Original language name

DNA-DOPE-gemini surfactants complexes at low surface charge density: from structure to transfection efficiency

Original language description

DNA condensation, structure and transfection efficiency of complexes formed by gemini surfactants alkane-alpha,omega-diyl-bis(dodecyldimethylammonium bromide)s (CnGS12, n = 3, 6 and 12 is the number of alkane spacer carbons), dioleoylphosphatidylethanolamine (CnGS12/DOPE = 0.3 mol/mol) and DNA at low surface charge density were investigated through different techniques. Small angle X-ray diffraction showed a condensed lamellar phase with marked dependence of DNA-DNA distance on (+/-) charge ratio. High ionic strength of hydrating medium screens the interaction DNA - CnGS12/DOPE and complexed DNA represented maximally ~ 45-60% of total DNA in the solution as derived from fluorescence and UV-VIS spectroscopy. The in vitro transfection efficiency of CnGS12/DOPE liposomes on mammalian HEK 293 cell line was spacer length-dependent. C12GS12/DOPE/DNA complexes exhibited the best transfection efficiency (~ 18% GFP-expressing cells relative to all viable cells) accompanied by ~ 89% cell viability.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30104 - Pharmacology and pharmacy

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

General Physiology and Biophysics

ISSN

0231-5882

e-ISSN

—

Volume of the periodical

37

Issue of the periodical within the volume

1

Country of publishing house

SK - SLOVAKIA

Number of pages

13

Pages from-to

57-69

UT code for WoS article

000425206200006

EID of the result in the Scopus database

2-s2.0-85044594233