Improved stability and efficacy of chitosan/pDNA complexes for gene delivery

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28610%2F15%3A43872977" target="_blank" >RIV/70883521:28610/15:43872977 - isvavai.cz</a>
Result on the web
<a href="http://link.springer.com/article/10.1007%2Fs10529-014-1727-7" target="_blank" >http://link.springer.com/article/10.1007%2Fs10529-014-1727-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10529-014-1727-7" target="_blank" >10.1007/s10529-014-1727-7</a>

Result language
angličtina
Original language name
Improved stability and efficacy of chitosan/pDNA complexes for gene delivery
Original language description
Among polymeric polycations, chitosan has emerged as a powerful carrier for gene delivery. Only a few studies have focused on the stability of the chitosan/DNA complex under storage, although this is imperative for nanomedicinal applications. Here, we synthesized polyelectrolyte complexes at a charge ratio of 10 using 50 kDa chitosan and plasmid (p)DNA that encodes a GFP reporter. These preparations were stable up to 3 months at 4 A degrees C and showed reproducible transfection efficiencies in vitro inHEK293 cells. In addition, we developed a methodology that increases the in vitro transfection efficiency of chitosan/pDNA complexes by 150 % with respect to standard procedures. Notably, intracellular pDNA release and transfected cells peaked 5 days following transection of mitotically active cells. These new developments in formulation technology enhance the potential for polymeric nanoparticle-mediated gene therapy.
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CD - Macromolecular chemistry
OECD FORD branch
—

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)