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Improved stability and efficacy of chitosan/pDNA complexes for gene delivery

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28610%2F15%3A43872977" target="_blank" >RIV/70883521:28610/15:43872977 - isvavai.cz</a>

  • Result on the web

    <a href="http://link.springer.com/article/10.1007%2Fs10529-014-1727-7" target="_blank" >http://link.springer.com/article/10.1007%2Fs10529-014-1727-7</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s10529-014-1727-7" target="_blank" >10.1007/s10529-014-1727-7</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Improved stability and efficacy of chitosan/pDNA complexes for gene delivery

  • Original language description

    Among polymeric polycations, chitosan has emerged as a powerful carrier for gene delivery. Only a few studies have focused on the stability of the chitosan/DNA complex under storage, although this is imperative for nanomedicinal applications. Here, we synthesized polyelectrolyte complexes at a charge ratio of 10 using 50 kDa chitosan and plasmid (p)DNA that encodes a GFP reporter. These preparations were stable up to 3 months at 4 A degrees C and showed reproducible transfection efficiencies in vitro inHEK293 cells. In addition, we developed a methodology that increases the in vitro transfection efficiency of chitosan/pDNA complexes by 150 % with respect to standard procedures. Notably, intracellular pDNA release and transfected cells peaked 5 days following transection of mitotically active cells. These new developments in formulation technology enhance the potential for polymeric nanoparticle-mediated gene therapy.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CD - Macromolecular chemistry

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Biotechnology Letters

  • ISSN

    0141-5492

  • e-ISSN

  • Volume of the periodical

    37

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    9

  • Pages from-to

    557-565

  • UT code for WoS article

    000350394800010

  • EID of the result in the Scopus database