In vitro and in vivo assessment of chitosan modified urocanic acid as gene carrier

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10361546" target="_blank" >RIV/00216208:11110/17:10361546 - isvavai.cz</a>

Alternative codes found

RIV/00064211:_____/17:W0000047

Result on the web

<a href="http://dx.doi.org/10.1016/j.msec.2016.09.024" target="_blank" >http://dx.doi.org/10.1016/j.msec.2016.09.024</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.msec.2016.09.024" target="_blank" >10.1016/j.msec.2016.09.024</a>

Result language

angličtina

Original language name

In vitro and in vivo assessment of chitosan modified urocanic acid as gene carrier

Original language description

Chitosan nanoparticles modified with 10 and 30% urocanic acid (CUA) via carbodiimide crosslinking were examined for an efficient gene delivery carrier. The CUA gene carrier was characterized by FTIR, TEM, SEM and the in vitro transfection efficiency CUA polyplex was tested with HeLa and 3T3 cells. The loading efficiency of CUA complexes with DNA was assessed at different N/P ratio of 1, 2, 4, 6, 8, and 10. The DNA loading efficiency was found be to &gt;85% for chitosan, CUA10 and CUA30% and the DNA protection ability of CUAl 0 and CUA30 nanoparticle complexes was confirmed upon incubation with Nhel and HindIII. The cell toxicity and cell viability results have supported the non-toxic nature of CUAl 0 and CUA30 nanoparticles. In vitro transfection efficiency of CUAl 0 and CUA30 polyplex was tested for EGFP expression in 3T3 and HeLa cells and a relative maximum % transfection of about 10% was confirmed by CUAl 0 and CUA30 after 96 h transfection. The feasibility and biocompatibility of CUA gene carrier in transgenic chickens was also demonstrated. The in vitro transfection and in vivo embryonic viability studies further confirmed the CUA as promising gene carrier because of the improved biocompatibility and DNA protection ability.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30212 - Surgery

Project

—

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Materials Science &amp; Engineering C

ISSN

0928-4931

e-ISSN

—

Volume of the periodical

70

Issue of the periodical within the volume

January

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

599-606

UT code for WoS article

000388046900071

EID of the result in the Scopus database

2-s2.0-84988637998