DNA intracellular delivery into 3T3 cell line using fluorescence magnetic ferumoxide nanoparticles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F18%3APU128180" target="_blank" >RIV/00216305:26220/18:PU128180 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/chapter/10.1007/978-981-10-9023-3_27" target="_blank" >https://link.springer.com/chapter/10.1007/978-981-10-9023-3_27</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-981-10-9023-3_27" target="_blank" >10.1007/978-981-10-9023-3_27</a>
Alternative languages
Result language
angličtina
Original language name
DNA intracellular delivery into 3T3 cell line using fluorescence magnetic ferumoxide nanoparticles
Original language description
Gene delivery is a widespread strategy in current experimental medicine. In this work, we report a method for low-toxic intracellular DNA vector delivery and post transfection localisation of this vector in mouse embryonic fibroblast cell lines. The surface of modified ferumoxide nanoparticles conjugated with Rhodamine B isothiocyanate (FeNV-Rh) was modified with linear polyethyleneimine and medium molecular weight chitosan to increase Accelerated Sensor of Action Potentials DNA vector adhesion. The size of the FeNV-Rh/DNA transfection complex was studied using dynamic light scattering (DLS) and scanning electron microscopy (SEM) techniques. The transfection complex internalisation of plasmid expression and FeNV-Rh, and stability of rhodamine fluorescence in intracellular space were observed at time periods 6, 12, 24 and 48 h post transfection. Results showed high transfection complex intracellular biocompatibility—cell viability after Rh-MNP labelling was higher than 97% 24 h after transfection, and higher than 95% after the next 24 h. Selective FeNV-Rh localisation in the lysosomes was quantified. More than 82% of nanoparticles were localised in the lysosomes 12 h post transfection and 94% of lysosomes had a significant and long-term deposit of nanoparticles. DNA vector expression was visible in >65% of the cells and precise protein localisation on the cell membrane was confirmed using confocal microscopy.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
—
OECD FORD branch
20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])
Result continuities
Project
<a href="/en/project/LO1401" target="_blank" >LO1401: Interdisciplinary Research of Wireless Technologies</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
World Congress on Medical Physics and Biomedical Engineering 2018
ISBN
978-981-10-9023-3
ISSN
1680-0737
e-ISSN
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Number of pages
5
Pages from-to
149-153
Publisher name
Springer
Place of publication
Singapore
Event location
Prague
Event date
Jun 3, 2018
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000449744300027