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MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F16%3APU120309" target="_blank" >RIV/00216305:26220/16:PU120309 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery

  • Original language description

    The non-toxic intracellular delivery of biopolymers is very hot topic in current biology. In this work, two different MATRA-A reagent/polyethyleneimine transfection protocols were used for intracellular delivery of ChR2 DNA vector. Cell morphology changes were studied 48 hours after transfection. Our transfection efficiency is lower than normally achieved, but the rate of cells with physiological properties remains is very high: 85.34%. Therefore, these transfection protocols are very applicable for electrophysiological experiments.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EI - Biotechnology and bionics

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    XVI. Workshop of Physical Chemists and Electrochemists

  • ISBN

    978-80-210-8267-0

  • ISSN

  • e-ISSN

  • Number of pages

    3

  • Pages from-to

    1-3

  • Publisher name

    Masarykova Univerzita - MUNI PRESS

  • Place of publication

    Brno, Czech Republic

  • Event location

    Brno

  • Event date

    Jun 6, 2016

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article