Imaging of transfection and intracellular release of intact, non-labeled DNA using fluorescent nanodiamonds
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F16%3A00471415" target="_blank" >RIV/68378271:_____/16:00471415 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/16:00471415 RIV/61389005:_____/16:00471415 RIV/61388963:_____/16:00471415 RIV/68407700:21460/16:00304392
Result on the web
<a href="http://dx.doi.org/10.1039/c6nr00610h" target="_blank" >http://dx.doi.org/10.1039/c6nr00610h</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c6nr00610h" target="_blank" >10.1039/c6nr00610h</a>
Alternative languages
Result language
angličtina
Original language name
Imaging of transfection and intracellular release of intact, non-labeled DNA using fluorescent nanodiamonds
Original language description
Efficient delivery of stabilized nucleic acids (NAs) into cells and release of the NA payload are crucial points in the transfection process. Here we report on the fabrication of a nanoscopic cellular delivery carrier that is additionally combined with a label-free intracellular sensor device, based on biocompatible fluorescent nanodiamond particles. The sensing function is engineered into nanodiamonds by using nitrogen-vacancy color centers, providing stable non-blinking luminescence. The device is used for monitoring NA transfection and the payload release in cells. The unpacking of NAs from a poly(ethyleneimine)-terminated nanodiamond surface is monitored using the color shift of nitrogen-vacancy centers in the diamond, which serve as a nanoscopic electric charge sensor.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BM - Solid-state physics and magnetism
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nanoscale
ISSN
2040-3364
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
23
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
12002-12012
UT code for WoS article
000378510700029
EID of the result in the Scopus database
2-s2.0-84974566680