Preparation of citrinin-selective molecularly imprinted polymer and its use for on-line solid-phase extraction coupled to liquid chromatography
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F19%3A10400790" target="_blank" >RIV/00216208:11160/19:10400790 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=C1zqKfo1G" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=C1zqKfo1G</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00216-019-01682-9" target="_blank" >10.1007/s00216-019-01682-9</a>
Alternative languages
Result language
angličtina
Original language name
Preparation of citrinin-selective molecularly imprinted polymer and its use for on-line solid-phase extraction coupled to liquid chromatography
Original language description
A new selective molecularly imprinted polymer has been prepared and used for extraction in on-line SPE-HPLC to achieve the selective determination of citrinin. Four different imprinted polymers varying in combinations of components were prepared by bulk polymerization and evaluated in terms of binding capacity and selectivity. Imprinted polymer prepared from a mixture comprising 1-hydoxy-2-naphtoic acid as the template molecule, acrylamide as the structural monomer, ethylene dimethacrylate as the cross-linker (in a molar ratio of 1:4:16), and acetonitrile as the porogenic solvent exhibited the best properties. The selectivity of this sorbent was confirmed by comparison with the non-imprinted counterpart prepared using the same polymerization carried out in the absence of template. Imprinted polymer was packed in a 20 x 3 mm i.d. steel cartridge and coupled to the on-line SPE-HPLC system through a six-port switching valve. The method for determination of citrinin including the on-line extraction step was then developed and validated. The sample in the form of methanolic extract was loaded, cleaned, and preconcentrated in the imprinted SPE cartridge. Subsequent separation of citrinin from residual interferences was achieved using the analytical column Kinetex Biphenyl 100 x 4.6 mm i.d., 5 m particle size, and fluorescence detection (Ex 335, Em 500 nm). The total analysis time was only 9.50 min. Our fully validated method was also applied to analysis of food supplements based on red yeast rice extracts, the control of which is implemented in European legislation. Only minor yet acceptable contamination was found in tested samples.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/EF16_019%2F0000841" target="_blank" >EF16_019/0000841: Efficiency and safety improvement of current drugs and nutraceuticals: advanced methods - new challenges</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical and Bioanalytical Chemistry
ISSN
1618-2642
e-ISSN
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Volume of the periodical
411
Issue of the periodical within the volume
11
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
2395-2404
UT code for WoS article
000464715500014
EID of the result in the Scopus database
2-s2.0-85061759425