Development of novel liquid chromatography method for clinical monitoring of vitamin B1 metabolites and B6 status in the whole blood
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F20%3A10407285" target="_blank" >RIV/00216208:11160/20:10407285 - isvavai.cz</a>
Alternative codes found
RIV/00179906:_____/20:10407285
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=PfsUeTHb9C" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=PfsUeTHb9C</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.talanta.2019.120702" target="_blank" >10.1016/j.talanta.2019.120702</a>
Alternative languages
Result language
angličtina
Original language name
Development of novel liquid chromatography method for clinical monitoring of vitamin B1 metabolites and B6 status in the whole blood
Original language description
A method for the simultaneous determination of vitamin B1 thiamine, and its phosphorylated biologically active derivatives thiamine diphosphate and thiamine monophosphate, together with the active form of vitamin B6 pyridoxal-5-phosphate in human whole blood has been developed and optimized using high performance liquid chromatography with pre-column derivatization coupled with fluorescence detection. The meteoric core column packed with core-shell stationary phase enabling separation using reversed phase mechanism was used for the analysis of real sample extracts and separation without the application of ion-pairing reagents was achieved. Recoveries were between 96.3% and 113.8% for all analytes. The analysis has quantitation limits suitable for clinical monitoring with LOQ values for pyridoxal-5-phosphate of 8.32 nmol/L, 4.35 nmol/L for thiamine diphosphate, 5.17 nmol/L for thiamine monophosphate, and 4.27 nmol/L for thiamine. Our method allowed determination and monitoring of trace levels of vitamins B1 and B6 in whole blood of patients receiving vitamin supplementation treatment while being in intensive care. Monitoring of their status was incorporated in clinical guidelines as an useful predictor of possible complications during treatment.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30104 - Pharmacology and pharmacy
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Talanta
ISSN
0039-9140
e-ISSN
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Volume of the periodical
211
Issue of the periodical within the volume
May
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
120702
UT code for WoS article
000518708300024
EID of the result in the Scopus database
2-s2.0-85077369797