Determination of pyridoxal, pyridoxal 5'-phosphate and 4-pyridoxic acid in plasma of selected groups of the patients with a view to proper sample preparation using HPLC with fluorescence detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F09%3A00008565" target="_blank" >RIV/00216275:25310/09:00008565 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Determination of pyridoxal, pyridoxal 5'-phosphate and 4-pyridoxic acid in plasma of selected groups of the patients with a view to proper sample preparation using HPLC with fluorescence detection

Original language description

A method for the measurement of pyridoxal, pyridoxal 5'-phosphate and 4-pyridoxic acid using a high-performance liquid chromatography with fluorescence detection with regard to stability and recovery is described. For the separation, a reverse phase column LiChroCart 125-4, Purospher RP-18e, 5 um, was used. The mixture of ethanol and 25 mmol/L Na2HPO4 (7:93, v/v), pH 6.0 was used as a mobile phase. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients ofvariation were below 10%. Quantitative recoveries from spiked plasma samples were at intervals 93.0-101.8%, except for pyridoxal (82.5-98.8%). The limit of detection was 1.0 nmol/L for pyridoxal 5'-phosphate, 0.7 nmol/L for 4-pyridoxic acid and 1.1 nmol/L for pyridoxal. The preliminary reference range of pyridoxal 5'-phosphate, pyridoxal and 4-pyridoxic acid in a group of blood donors are 42.0-75.6 nmol/L, 2.6-12.8 nmol/L and 16.2-33.0 nmol/L, respectively.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

—

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Papers of the University of Pardubice, Series A

ISSN

1211-5541

e-ISSN

—

Volume of the periodical

15

Issue of the periodical within the volume

12

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

13

Pages from-to

—

UT code for WoS article

—

EID of the result in the Scopus database

—