Determination of 25-hydroxyvitamin D3 in human plasma using HPLC with UV detection based on SPE sample preparation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F09%3A00008562" target="_blank" >RIV/00216275:25310/09:00008562 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Determination of 25-hydroxyvitamin D3 in human plasma using HPLC with UV detection based on SPE sample preparation
Original language description
Interest in the metabolism and physiological action of vitamin D is increased exponentially. The most important metabolites of vitamin D are 25-hydroxyvitamin and 1,25-dihydroxyvitamin D3. The aim of the study was to develop a rapid and simple HPLC method for the measurement of 25-hydroxyvitamin D3 in human plasma. A method for the measurement of 25-hydroxyvitamin D3 using HPLC with UV detection and investigation into the extraction techniques with regard to stability and recovery are described. For theseparation, RP column LiChroCart 125-4, Purospher RP-18e, 5 um, was used. The mixture of methanol and deionized water (95:5 v/v) was used as mobile phase. The analytical performance of this method is satisfactory: the intra- and inter-assay coefficientsof variation were below 10%. Quantitative recoveries from spiked plasma samples were between 92.0-103.2%. The LOD was 10 nmol/L. The preliminary reference range of 25-hydroxyvitamin D3 in a group of blood donors is 62 +/- 26 nmol/L.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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