The power of modern extraction techniques: A breakthrough in vitamin K extraction from human serum

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F24%3A10481712" target="_blank" >RIV/00216208:11160/24:10481712 - isvavai.cz</a>

Alternative codes found

RIV/00179906:_____/24:10481712

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=jp6_eQCg4X" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=jp6_eQCg4X</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2024.110170" target="_blank" >10.1016/j.microc.2024.110170</a>

Result language

angličtina

Original language name

The power of modern extraction techniques: A breakthrough in vitamin K extraction from human serum

Original language description

As a representative of the lipophilic vitamins, vitamin K is one of the most difficult vitamins to analyze. Even modern approaches using mass spectrometry detection cannot be carried out without multi-step, time-consuming, economically, and ecologically demanding sample treatment. These findings suggest an opportunity to develop new modern trends that could shorten and simplify the whole procedure, whereby emphasizing the processing of a large number of samples with an additional focus on more environmentally friendly techniques. We selected and compared the main types of both, modern and conventional approaches for vitamin K analysis in human serum including liquid-liquid extraction and solid-phase extraction on the one hand, as well as supported liquid extraction, PhreeTM phospholipid removal plate, miniaturized liquid-liquid extraction, and dispersive pipette extraction on the other hand. The different techniques were compared from an analytical perspective, including parameters such as recovery, limits of detection, and matrix effects, as well as from a non-analytical perspective, including time consumption, cost effectiveness, and manual labor required to perform the procedure. The modern techniques seem to have great advantages. Our results indicate that the PhreeTM phospholipid removal plate, and the miniaturized version of the liquid-liquid extraction are the best techniques. This work was also carried out to provide a guide for the extraction of vitamin K while determining it using the liquid chromatography-mass spectrometry technique, which may improve sample handling and help to remove potential obstacles in the future.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30100 - Basic medicine

Project

<a href="/en/project/NU21J-02-00021" target="_blank" >NU21J-02-00021: Differences in parameters of platelets aggregation and blood coagulation between healthy individuals and patients with metabolic diseases</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microchemical Journal

ISSN

0026-265X

e-ISSN

1095-9149

Volume of the periodical

198

Issue of the periodical within the volume

MAR

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

10

Pages from-to

110170

UT code for WoS article

001203047400001

EID of the result in the Scopus database

2-s2.0-85187300798