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Prp45 Affects Prp22 Partition in Spliceosomal Complexes and Splicing Efficiency of Non-Consensus Substrates

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F09%3A00013036" target="_blank" >RIV/00216208:11310/09:00013036 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Prp45 Affects Prp22 Partition in Spliceosomal Complexes and Splicing Efficiency of Non-Consensus Substrates

  • Original language description

    Prp45 is an yeast ortholog of Human transcription co-regulator SNW1/SKIP, which is implicated in the regulation of both transcription elongation and alternative splicing. We characterize a temperature sensitive allele of PRP45 using a synthetic lethalityscreen, TAP purification and primer extension splicing assay. We found that prp45(1?169) genetically interacts spliceosomal components SYF1, CLF1/SYF3, NTC20, and CEF1, SLU7, PRP17, PRP18, and PRP22. Spliceosomal complexes purified from prp45(1?169) cells showed decreased stoichiometry of Prp22, suggesting its deranged interaction with the spliceosome. Splicing of canonical intron was unimpeded, wheras mutants showed elevated levels of lariatexon intermediate. We suggest that Prp45 contributes to splicing efficiency of substrates non-conforming to the consensus.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2009

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Cellular Biochemistry

  • ISSN

    0730-2312

  • e-ISSN

  • Volume of the periodical

    106

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    13

  • Pages from-to

  • UT code for WoS article

    000262238600016

  • EID of the result in the Scopus database