Prp45 Affects Prp22 Partition in Spliceosomal Complexes and Splicing Efficiency of Non-Consensus Substrates
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F09%3A00013036" target="_blank" >RIV/00216208:11310/09:00013036 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Prp45 Affects Prp22 Partition in Spliceosomal Complexes and Splicing Efficiency of Non-Consensus Substrates
Original language description
Prp45 is an yeast ortholog of Human transcription co-regulator SNW1/SKIP, which is implicated in the regulation of both transcription elongation and alternative splicing. We characterize a temperature sensitive allele of PRP45 using a synthetic lethalityscreen, TAP purification and primer extension splicing assay. We found that prp45(1?169) genetically interacts spliceosomal components SYF1, CLF1/SYF3, NTC20, and CEF1, SLU7, PRP17, PRP18, and PRP22. Spliceosomal complexes purified from prp45(1?169) cells showed decreased stoichiometry of Prp22, suggesting its deranged interaction with the spliceosome. Splicing of canonical intron was unimpeded, wheras mutants showed elevated levels of lariatexon intermediate. We suggest that Prp45 contributes to splicing efficiency of substrates non-conforming to the consensus.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Cellular Biochemistry
ISSN
0730-2312
e-ISSN
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Volume of the periodical
106
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
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UT code for WoS article
000262238600016
EID of the result in the Scopus database
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