Truncating the spliceosomal 'rope protein' Prp45 results in Htz1 dependent phenotypes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00603215" target="_blank" >RIV/60077344:_____/24:00603215 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/24:10487127

Result on the web

<a href="https://doi.org/10.1080/15476286.2024.2348896" target="_blank" >https://doi.org/10.1080/15476286.2024.2348896</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/15476286.2024.2348896" target="_blank" >10.1080/15476286.2024.2348896</a>

Result language

angličtina

Original language name

Truncating the spliceosomal 'rope protein' Prp45 results in Htz1 dependent phenotypes

Original language description

Spliceosome assembly contributes an important but incompletely understood aspect of splicing regulation. Prp45 is a yeast splicing factor which runs as an extended fold through the spliceosome, and which may be important for bringing its components together. We performed a whole genome analysis of the genetic interaction network of the truncated allele of PRP45 (prp45(1-169)) using synthetic genetic array technology and found chromatin remodellers and modifiers as an enriched category. In agreement with related studies, H2A.Z-encoding HTZ1, and the components of SWR1, INO80, and SAGA complexes represented prominent interactors, with htz1 conferring the strongest growth defect. Because the truncation of Prp45 disproportionately affected low copy number transcripts of intron-containing genes, we prepared strains carrying intronless versions of SRB2, VPS75, or HRB1, the most affected cases with transcription-related function. Intron removal from SRB2, but not from the other genes, partly repaired some but not all the growth phenotypes identified in the genetic screen. The interaction of prp45(1-169) and htz1 Delta was detectable even in cells with SRB2 intron deleted (srb2 Delta i). The less truncated variant, prp45(1-330), had a synthetic growth defect with htz1 Delta at 16 degrees C, which also persisted in the srb2 Delta i background. Moreover, htz1 Delta enhanced prp45(1-330) dependent pre-mRNA hyper-accumulation of both high and low efficiency splicers, genes ECM33 and COF1, respectively. We conclude that while the expression defects of low expression intron-containing genes contribute to the genetic interactome of prp45(1-169), the genetic interactions between prp45 and htz1 alleles demonstrate the sensitivity of spliceosome assembly, delayed in prp45(1-169), to the chromatin environment.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

RNA biology

ISSN

1547-6286

e-ISSN

1555-8584

Volume of the periodical

21

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

18

Pages from-to

543-559

UT code for WoS article

001237152800001

EID of the result in the Scopus database

2-s2.0-85192166077