Residual concentration of estriol during mouse sperm capacitation in vitro determined by HPLC method

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F09%3A10000954" target="_blank" >RIV/00216208:11310/09:10000954 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Residual concentration of estriol during mouse sperm capacitation in vitro determined by HPLC method

Original language description

The aim of this work was to develop a method for determining free estriol available to capacitating sperm. In order to determine a status of estriol during mouse sperm capacitation in vitro a high performance liquid chromatography HPLC method with UV detection was used. A free estriol, and the estriol bound to the bovine serum albumin in capacitation medium can be quantified by the proposed method. A reversed-phase separation mode using a SunFire C18 column with a simple mobile phase composed of acetonitrile and water, methanol and water at the ratio 40/60 (v/v) was applied. Our results show that the level of free estriol available for mouse spermatozoa during capacitation in vitro can be quantified by HPLC method with UV detection. Therefore, this method represents an important tool to determine the amount of environmental estrogens, such as estriol, bound to sperm cells at the specific time point of capacitation in vitro.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EG - Zoology

OECD FORD branch

—

Project

<a href="/en/project/1M06011" target="_blank" >1M06011: The Centre of Molecular Methods for Monitoring the Diffuse Pollution of the Environment.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FOLIA ZOOLOGICA

ISSN

0139-7893

e-ISSN

—

Volume of the periodical

58

Issue of the periodical within the volume

Suppl 1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

—

UT code for WoS article

000272048200008

EID of the result in the Scopus database

—