A PCR method of detecting American Foulbrood (Paenibacillus larvae) in winter beehive wax debris

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F09%3A10001170" target="_blank" >RIV/00216208:11310/09:10001170 - isvavai.cz</a>
Alternative codes found
RIV/60460709:41210/09:31056
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
A PCR method of detecting American Foulbrood (Paenibacillus larvae) in winter beehive wax debris
Original language description
The objective of this work was to create a fast and sensitive method of detecting Paenibacillus larvae from beehive debris based on PCR that does not require long-lasting cultivation steps. Various methods of extracting spores from beehive debris were compared: the original method of extraction of spores into toluene, and alternative spore extraction methods into Tween 80, into water, into isopropanol and into 95% ethanol. Isolation of DNA from various spore extractions was evaluated too. Best results were provided by isolation of DNA using the QIAamp DNA Mini Kit, without heat treatment. DNA of spores was detected by PCR from 0.25 g of beeswax debris, with the detected titer of 105 in 1 g according to the cultivation tests.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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