High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10100469" target="_blank" >RIV/00216208:11310/11:10100469 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/11:00369717
Result on the web
<a href="http://dx.doi.org/10.1016/j.pep.2011.01.013" target="_blank" >http://dx.doi.org/10.1016/j.pep.2011.01.013</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.pep.2011.01.013" target="_blank" >10.1016/j.pep.2011.01.013</a>
Alternative languages
Result language
angličtina
Original language name
High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli
Original language description
Mouse NKR-P1C(B6) receptor corresponding to NK1.1 alloantigen is one of the most widespread surfacemarkers of mouse NK and NKT cells in C57BL/6 mice detected by monoclonal antibody PK136. Althoughfunctional studies revealed the ability of this receptor to activate both natural killing and production ofcytokines upon antibody crosslinking, the ligand for NKR-P1C(B6) remains unknown. In order to initiate ligand identification, structural studies, and epitope mapping experiments, we developed a simple andefficient expression and purification protocol allowing to produce large amounts of pure soluble monomeric mouse NKR-P1C(B6). Our protein encompassed approximately half of the stalk region and the entire C-terminal globular ligand binding domain.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EC - Immunology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Protein Expression and Purification
ISSN
1046-5928
e-ISSN
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Volume of the periodical
77
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
178-184
UT code for WoS article
000288591900008
EID of the result in the Scopus database
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