Polyomavirus Middle T-Antigen Is a Transmembrane Protein That Binds Signaling Proteins in Discrete Subcellular Membrane Sites

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10106664" target="_blank" >RIV/00216208:11310/11:10106664 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1128/JVI.02209-10" target="_blank" >http://dx.doi.org/10.1128/JVI.02209-10</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/JVI.02209-10" target="_blank" >10.1128/JVI.02209-10</a>

Result language
angličtina
Original language name
Polyomavirus Middle T-Antigen Is a Transmembrane Protein That Binds Signaling Proteins in Discrete Subcellular Membrane Sites
Original language description
Murine polyomavirus middle T-antigen (MT) induces tumors by mimicking an activated growth factor receptor. An essential component of this action is a 22-amino-acid hydrophobic region close to the C terminus which locates MT to cell membranes. Here, we demonstrate that this sequence is a transmembrane domain (TMD) by showing that a hemagglutinin (HA) tag added to the MT C terminus is exposed on the outside of the cells, with the N terminus inside. The mutant protein with addition of the ER retention signal KDEL to the MT C terminus, locates only in the ER, demonstrating that MT does insert into membranes solely at this location. In addition, this ER-located MT failed to transform. MTKDEL protein failed to interact with ShcA, phosphatidylinositol 3-kinase (PI3K), and phospholipase C-1 (PLC-1), despite being tyrosine phosphorylated. Additional studies showed that MT binding to PP2A is probably required for MT to ef?ciently exit the ER and migrate to the plasma membrane. Overall, these dat
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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