Purine P2Y receptors in ATP-mediated regulation of non-quantal acetylcholine release from motor nerve endings of rat diaphragm

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10106758" target="_blank" >RIV/00216208:11310/11:10106758 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.neures.2011.07.1829" target="_blank" >http://dx.doi.org/10.1016/j.neures.2011.07.1829</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.neures.2011.07.1829" target="_blank" >10.1016/j.neures.2011.07.1829</a>

Result language

angličtina

Original language name

Purine P2Y receptors in ATP-mediated regulation of non-quantal acetylcholine release from motor nerve endings of rat diaphragm

Original language description

We established the effect of ATP, which is released together with acetylcholine (ACh), on the non-quantal ACh release (NQR) in rat diaphragm endplates and checked what kind of purine receptors are involved. NQR was estimated by the amplitude of endplatehyperpolarization (the H-effect) following the blockade of postsynaptic nicotinic receptors and cholinesterase. 100 ?M ATP reduced the H-effect to 66% of the control. The action of ATP remained unchanged after the inhibition of ionotropic P2X receptors by Evans blue and PPADS, but disappeared after the application of the broad spectrum P2 receptor antagonist suramin, metabotropic P2Y receptor blocker reactive blue 2 and U73122, an inhibitor of phospholipase C. P2Y-mediated regulation is not coupled to presynaptic voltage-dependent Ca(2+) channels. During the s?taneous application of ATP and glutamate (which is another ACh cotransmitter reducing non-quantal release), the additive depressant effect led to a disappearance of the H-effect.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Neuroscience Research

ISSN

0168-0102

e-ISSN

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Volume of the periodical

71

Issue of the periodical within the volume

3

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

219-225

UT code for WoS article

000295956800004

EID of the result in the Scopus database

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