Native polyacrylamide electrophoresis in the presence of Ponceau Red to study oligomeric states of protein complexes.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10107525" target="_blank" >RIV/00216208:11310/11:10107525 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/jssc.201000869" target="_blank" >http://dx.doi.org/10.1002/jssc.201000869</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.201000869" target="_blank" >10.1002/jssc.201000869</a>
Alternative languages
Result language
angličtina
Original language name
Native polyacrylamide electrophoresis in the presence of Ponceau Red to study oligomeric states of protein complexes.
Original language description
Native polyacrylamide electrophoresis in the presence of two reversible protein anionic stains (Ponceau S and Ponceau 2R) was used to study the oligomeric states of soluble proteins. A mild binding of the used protein stains to nondissociated protein oligomers imposed a charge shift on the proteins resulting into separation of protein species according to their size under physiological conditions. Adsorbed stains could be easily removed after electrophoresis by washing of polyacrylamide gel with bufferand protein complexes could be visualized either by the detection of their enzyme activity or by using a nonspecific protein stain. The specific detection of enzyme activity of glycosidases, lactate dehydrogenase, or phosphatases was shown as an example.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA303%2F09%2F1285" target="_blank" >GA303/09/1285: Study of the sperm membrane proteins with zona pellucida binding activity</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Separation Science
ISSN
1615-9306
e-ISSN
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Volume of the periodical
34
Issue of the periodical within the volume
14
Country of publishing house
GB - UNITED KINGDOM
Number of pages
4
Pages from-to
1692-1695
UT code for WoS article
000293588900009
EID of the result in the Scopus database
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