Native Red Electrophoresis - A new method suitable for separation of native proteins
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10107538" target="_blank" >RIV/00216208:11310/11:10107538 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/11:00371665
Result on the web
<a href="http://dx.doi.org/10.1002/elps.201100310" target="_blank" >http://dx.doi.org/10.1002/elps.201100310</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201100310" target="_blank" >10.1002/elps.201100310</a>
Alternative languages
Result language
angličtina
Original language name
Native Red Electrophoresis - A new method suitable for separation of native proteins
Original language description
A new type of native electrophoresis was developed to separate and characterize proteins. In this modi?cation of the native blue electrophoresis, the dye Ponceau Red S is used instead of Coomassie Brilliant Blue to impose uniform negative charge on proteins to enable their electrophoretic separation according to their relative molecular masses. As Ponceau Red S binds less tightly to proteins, in comparison with Coomassie Blue, it can be easily removed after the electrophoretic separation and a further investigation of protein properties is made possible (e.g. an enzyme detection or electroblotting). The tested proteins also kept their native properties (enzyme activity or aggregation state).
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA303%2F09%2F1285" target="_blank" >GA303/09/1285: Study of the sperm membrane proteins with zona pellucida binding activity</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
32
Issue of the periodical within the volume
24
Country of publishing house
GB - UNITED KINGDOM
Number of pages
3
Pages from-to
3597-3599
UT code for WoS article
000298587900016
EID of the result in the Scopus database
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