Visualisation of Leishmania donovani Fluorescent Hybrids during Early Stage Development in the Sand Fly Vector
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10108261" target="_blank" >RIV/00216208:11310/11:10108261 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1371/journal.pone.0019851" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0019851</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0019851" target="_blank" >10.1371/journal.pone.0019851</a>
Alternative languages
Result language
angličtina
Original language name
Visualisation of Leishmania donovani Fluorescent Hybrids during Early Stage Development in the Sand Fly Vector
Original language description
Background: The Leishmania protozoan parasites cause devastating human diseases. Leishmania have been considered to replicate clonally, without genetic exchange. However, an accumulation of evidence indicates that there are inter-specific and intra-specific hybrids among natural populations. The first and so far only experimental proof of genetic exchange was obtained in 2009 when double drug resistant Leishmania major hybrids were produced by co-infecting sand flies with two strains carrying differentdrug resistance markers. However, the location and timing of hybridisation events in sand flies has not been described. Methodology/Principal Findings: Here we have co-infected Phlebotomus perniciosus and Lutzomyia longipalpis with transgenic promastigotes of Leishmania donovani strains carrying hygromycin or neomycin resistance genes and red or green fluorescent markers. Fed females were dissected at different times post bloodmeal (PBM) and examined by fluorescent microscopy or fluoresc
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EG - Zoology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LC06009" target="_blank" >LC06009: Centre for molecular ecology of vectors and pathogens</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS ONE
ISSN
1932-6203
e-ISSN
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Volume of the periodical
6
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
"e19851", 1-9
UT code for WoS article
000291052500014
EID of the result in the Scopus database
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