Preparation of highly infective Leishmania promastigotes by cultivation on SNB-9 biphasic medium

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10108322" target="_blank" >RIV/00216208:11310/11:10108322 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/11:00371215
Result on the web
<a href="http://dx.doi.org/10.1016/j.mimet.2011.08.012" target="_blank" >http://dx.doi.org/10.1016/j.mimet.2011.08.012</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.mimet.2011.08.012" target="_blank" >10.1016/j.mimet.2011.08.012</a>

Result language
angličtina
Original language name
Preparation of highly infective Leishmania promastigotes by cultivation on SNB-9 biphasic medium
Original language description
Protozoan hemoflagellates Leishmania are causative agents of leishmaniases and an important biological model for study of host-pathogen interaction. A wide range of methods of Leishmania cultivation on both biphasic and liquid media is available. Biphasic media are considered to be superior for initial isolation of the parasites and obtaining high promastigote infectivity; however, liquid media are more suitable for large-scale experiments. The aim of the present study was the adaptation and optimization of the cultivation of Leishmania promastigotes on a biphasic SNB-9 (saline-neopeptone-blood 9) medium that was originally developed for Trypanosoma cultivation and combines the advantages of biphasic and liquid media. SNB-9 medium is characterized witha large volume of the liquid phase, which facilitates the manipulation with the culture and provides parasite yields comparable to parasite yields on such liquid medium as Schneider&amp;apos;s Insect Medium. We demonstrate that SNB-9
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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