Purification and characterization of heterologously expressed nitrilases from filamentous fungi

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10104142" target="_blank" >RIV/00216208:11310/12:10104142 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/12:00377497

Result on the web

<a href="http://www.springerlink.com/content/ukq6182w1754707u/fulltext.pdf" target="_blank" >http://www.springerlink.com/content/ukq6182w1754707u/fulltext.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00253-011-3525-7" target="_blank" >10.1007/s00253-011-3525-7</a>

Result language

angličtina

Original language name

Purification and characterization of heterologously expressed nitrilases from filamentous fungi

Original language description

Nitrilases from Aspergillus niger CBS 513.88, A. niger K10, Gibberella moniliformis, Neurospora crassa OR74A, and Penicillium marneffei ATCC 18224 were expressed in Escherichia coli BL21-Gold (DE3) after IPTG induction. N. crassa nitrilase exhibited thehighest yield of 69,000 ULMINUS SIGN 1 culture. Co-expression of chaperones (GroEL/ES in G. moniliformis and P. marneffei; GroEL/ ES and trigger factor in N. crassa and A. niger CBS 513.88) enhanced the enzyme solubility. Specific activities of strains expressing the former two enzymes increased approximately fourfold upon co-expression of GroEL/ES. The enzyme from G. moniliformis (co-purified with GroEL) preferred benzonitrile as substrate (Km of 0.41 mM, Vmax of 9.7 ?mol minMINUS SIGN 1 mgMINUS SIGN 1protein). The P. marneffei enzyme (unstable in its purified state) exhibited the highest Vmax of 7.3 ?mol minMINUS SIGN 1 mgMINUS SIGN 1 protein in cellfree extract, but also a high Km of 15.4 mM, for 4- cyanopyridine. The purified nitri

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Applied Microbiology and Biotechnology

ISSN

0175-7598

e-ISSN

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Volume of the periodical

93

Issue of the periodical within the volume

4

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

1553-1561

UT code for WoS article

000300310100018

EID of the result in the Scopus database

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