Genome mining for the discovery of new nitrilases in filamentous fungi
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10062490" target="_blank" >RIV/00216208:11310/11:10062490 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/11:00360696
Result on the web
<a href="http://dx.doi.org/10.1007/s10529-010-0421-7" target="_blank" >http://dx.doi.org/10.1007/s10529-010-0421-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10529-010-0421-7" target="_blank" >10.1007/s10529-010-0421-7</a>
Alternative languages
Result language
angličtina
Original language name
Genome mining for the discovery of new nitrilases in filamentous fungi
Original language description
Our aim is to describe new fungal nitrilases whose sequences were published but whose catalytic properties were unknown. We adapted for expression in E. coli three of the genes and confirmed that the enzymes acted on organic nitriles. The genome mining approach was used to search for nitrilases in filamentous fungi. Synthetic genes encoding nitrilases in Aspergillus niger, Gibberella moniliformis and Neurospora crassa were expresse in Escherichia coli. This is the first heterologous expression of fungalenzymes of this type. The recombinant enzyme derived from G. Moniliformis was an aromatic nitrilase with an activity of 390 U l-1 culture with benzonitrile as substrate. This was much less than the activities of the recombinant enzymes derived from A.niger and N. crassa that had activities of 2500 and 2700 U l-1 culture, respectively, with phenylacetonitrile as substrate.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biotechnology Letters
ISSN
0141-5492
e-ISSN
—
Volume of the periodical
33
Issue of the periodical within the volume
2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
4
Pages from-to
309-312
UT code for WoS article
000286198700014
EID of the result in the Scopus database
—