Bringing nitrilase sequences from databases to life: the search for novel substrate specificities with a focus on dinitriles

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F16%3A00461367" target="_blank" >RIV/61389013:_____/16:00461367 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/16:00460036 RIV/00216208:11310/16:10334550
Result on the web
<a href="http://dx.doi.org/10.1007/s00253-015-7023-1" target="_blank" >http://dx.doi.org/10.1007/s00253-015-7023-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00253-015-7023-1" target="_blank" >10.1007/s00253-015-7023-1</a>

Result language
angličtina
Original language name
Bringing nitrilase sequences from databases to life: the search for novel substrate specificities with a focus on dinitriles
Original language description
The aim of this study was to discover new nitrilases with useful activities, especially towards dinitriles that are precursors of high-value cyano acids. Genes coding for putative nitrilases of different origins (fungal, plant, or bacterial) with moderate similarities to known nitrilases were selected by mining the GenBank database, synthesized artificially and expressed in Escherichia coli. The enzymes were purified, examined for their substrate specificities, and classified into subtypes (aromatic nitrilase, arylacetonitrilase, aliphatic nitrilase, cyanide hydratase) which were largely in accordance with those predicted from bioinformatic analysis. The catalytic potential of the nitrilases for dinitriles was examined with cyanophenyl acetonitriles, phenylenediacetonitriles, and fumaronitrile. The nitrilase activities and selectivities for dinitriles and the reaction products (cyano acid, cyano amide, diacid) depended on the enzyme subtype. At a preparative scale, all the examined dinitriles were hydrolyzed into cyano acids and fumaronitrile was converted to cyano amide using E. coli cells producing arylacetonitrilases and an aromatic nitrilase, respectively.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CD - Macromolecular chemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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