Recent advances and challenges in the heterologous production of microbial nitrilases for biocatalytic applications

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00473528" target="_blank" >RIV/61388971:_____/17:00473528 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s11274-016-2173-6" target="_blank" >http://dx.doi.org/10.1007/s11274-016-2173-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11274-016-2173-6" target="_blank" >10.1007/s11274-016-2173-6</a>

Result language

angličtina

Original language name

Recent advances and challenges in the heterologous production of microbial nitrilases for biocatalytic applications

Original language description

The aim of this study is to review the current state of and highlight the challenges in the production of microbial nitrilases as catalysts for the mild hydrolysis of industrially important nitriles. Together with aldoxime dehydratase, the nitrile-hydrolyzing enzymes (nitrilase, nitrile hydratase) are key enzymes in the aldoxime-nitrile pathway which is widely distributed in bacteria and fungi. The availability of nitrilases has grown significantly over the past decade due to the use of metagenomic and database-mining approaches. Databases contain plenty of putative enzymes of this type, whose overproduction may improve the spectrum and the industrial utility of nitrilases. By exploiting this resource, the number of experimentally verified nitrilases has recently increased to several hundred. We especially focus on the efficient heterologous expression systems that are applicable for the overproduction of wild-type nitrilases and their artificial variants. Biocatalyst forms with industrial potential are also highlighted. The potential industrial applications of nitrilases are classified according to their target products (a-hydroxy acids, a-and beta-amino acids, cyano acids, amides). The emerging uses of nitrilases and their subtypes (cyanide hydratases, cyanide dihydratases) in bioremediation is also summarized. The integration of nitrilases with other enzymes into artificial multienzymatic and chemoenzymatic pathways is considered a promising strategy for future applications.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/LD15107" target="_blank" >LD15107: New aldoxime dehydratases: isolation, characterization and use in biocatalytic modules</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

World Journal of Microbiology and Biotechnology

ISSN

0959-3993

e-ISSN

—

Volume of the periodical

33

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

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UT code for WoS article

000392115600008

EID of the result in the Scopus database

2-s2.0-84995772487