Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10120331" target="_blank" >RIV/00216208:11310/12:10120331 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/12:00381851 RIV/61388955:_____/12:00381851

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S0005273612001538" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0005273612001538</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbamem.2012.05.005" target="_blank" >10.1016/j.bbamem.2012.05.005</a>

Result language

angličtina

Original language name

Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM)

Original language description

Each of the three plasma membrane Ato proteins is involved in ammonium signalling and the development of yeast colonies. This suggests that although these proteins are homologous, they do not functionally substitute for each other, but may forma functional complex. Here,we present a detailed combined FRET, FLIM and photobleaching study, which enabled us to detect interactions between Ato proteins found in distinct compartments of yeast cells. We thus show that the proteins Ato1p and Ato2p interact and can form complexes when present in the plasmamembrane. No interaction was detected between Ato1p and Ato3p or Ato2p and Ato3p. In addition, using specially prepared strains, we were able to detect an interaction between molecules of the same Ato protein,namely Ato1p-Ato1p and Ato3p-Ato3p, but not Ato2p-Ato2p.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochimica et Biophysica Acta - Biomembranes

ISSN

0005-2736

e-ISSN

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Volume of the periodical

1818

Issue of the periodical within the volume

9

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

2126-2134

UT code for WoS article

000306882600006

EID of the result in the Scopus database

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