Simple colorimetric methods for determination of sub-milligram amounts of ultrahigh molecular weight polyethylene wear particles

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10120333" target="_blank" >RIV/00216208:11310/12:10120333 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/12:00377013 RIV/61388963:_____/12:00377013 RIV/00064203:_____/12:7976 RIV/00216208:11110/12:10120333
Result on the web
<a href="http://dx.doi.org/10.1016/j.actbio.2012.01.010" target="_blank" >http://dx.doi.org/10.1016/j.actbio.2012.01.010</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.actbio.2012.01.010" target="_blank" >10.1016/j.actbio.2012.01.010</a>

Result language
angličtina
Original language name
Simple colorimetric methods for determination of sub-milligram amounts of ultrahigh molecular weight polyethylene wear particles
Original language description
New colorimetric methods are described for determination of sub-milligram amounts of ultra-high molecular weight polyethylene (UHMWPE) wear particles. These methods are based on the irreversible binding of the fluorescein-conjugated bovine serum albuminor the hydrophobic dye Oil Red O to wear particles. UHMWPE particles bind both substances from their solutions and thus decrease the absorbance of these solutions. The decrease is linearly dependent on the amount of added wear particles in the sub-milligram range suitable for practical use. The newly developed method offers improved accuracy and precision compared to Fourier transformed infrared spectroscopy (Slouf M. et al. Quantification of UHMWPE wear in periprosthetic tissues of hip arthoplasty: description of a new method based on IR and comparison with radiographic appearance.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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