Transient expression of Human papillomavirus type 16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in plants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10123194" target="_blank" >RIV/00216208:11310/12:10123194 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/12:00380685 RIV/00023736:_____/12:00009612

Result on the web

<a href="http://dx.doi.org/10.1007/s12038-011-9177-z" target="_blank" >http://dx.doi.org/10.1007/s12038-011-9177-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12038-011-9177-z" target="_blank" >10.1007/s12038-011-9177-z</a>

Result language

angličtina

Original language name

Transient expression of Human papillomavirus type 16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in plants

Original language description

Transient expression of foreign genes based on plant viral vectors is a suitable system for the production of relevant immunogens that can be used for the development of a new generation of vaccines against a variety of infectious diseases. In the present study the epitope derived from HPV-16 L2 minor capsid protein (amino acids 108-120) was expressed from Potato virus X (PVX)-based vector pGR106 as N- or C-terminal fusion with the PVX coat protein (PVX CP) in transgenic Nicotiana benthamiana plants. The fusion protein L2(108-120)-PVX CP was successfully expressed in plants at a level of 170 mg/kg of fresh leaf tissue. The C-terminal fusion protein PVX CP- L2(108-120) was expressed using mutated vector sequence to avoid homologous recombination at a level of 8 mg/kg of fresh leaf tissue. Immunogenicity of L2(108-120)-PVX CP virus-like particles was tested after immunization of mice by subcutaneous injection or tattoo administration. In animal sera the antibodies against the PVX CP and

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biosciences

ISSN

0250-5991

e-ISSN

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Volume of the periodical

37

Issue of the periodical within the volume

1

Country of publishing house

IN - INDIA

Number of pages

9

Pages from-to

125-133

UT code for WoS article

000301163400015

EID of the result in the Scopus database

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