A GoldenBraid-compatible virus-based vector system for transient expression of heterologous proteins in plants
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F22%3A94611" target="_blank" >RIV/60460709:41210/22:94611 - isvavai.cz</a>
Alternative codes found
RIV/61389030:_____/22:00561488 RIV/00216208:11310/22:10445326 RIV/00027006:_____/22:10175664
Result on the web
<a href="https://www.mdpi.com/1999-4915/14/5/1099" target="_blank" >https://www.mdpi.com/1999-4915/14/5/1099</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/v14051099" target="_blank" >10.3390/v14051099</a>
Alternative languages
Result language
angličtina
Original language name
A GoldenBraid-compatible virus-based vector system for transient expression of heterologous proteins in plants
Original language description
We have developed a Potato virus X (PVX)-based vector system compatible with the GoldenBraid 2,0 (GB) cloning strategy to transiently express heterologous proteins or peptides in plants for biotechnological purposes. This vector system consists of three domestication vectors carrying three GB parts the cauliflower mosaic virus (CaMV) 35S promoter with PVX upstream of the second subgenomic promoter of the PVX coat protein (PVX CP SGP), nopaline synthase (NOS) terminator with PVX downstream of the first PVX CP SGP and the gene of interest (GOI). The full-length PVX clone carrying the sequence encoding a green fluorescent protein (GFP) as GOI was incorporated into the binary GB vector in a one-step reaction of three GB parts using the four-nucleotide GB standard syntax. We investigated whether the obtained vector named GFP/pGBX enables systemic PVX infection and expression of GFP in Nicotiana benthamiana plants. We show that this GB-compatible vector system can be used for simple and efficient assembly of PVX-based expression constructs and that it meets the current need for interchange of standard biological parts used in different expression systems.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Viruses-Basel
ISSN
1999-4915
e-ISSN
1999-4915
Volume of the periodical
14
Issue of the periodical within the volume
5
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
14
Pages from-to
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UT code for WoS article
000801340000001
EID of the result in the Scopus database
2-s2.0-85130966974