Potato virus X displaying the E7 peptide derived from human papillomavirus type 16: a novel position for epitope presentation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10294229" target="_blank" >RIV/00216208:11310/15:10294229 - isvavai.cz</a>
Alternative codes found
RIV/61389030:_____/15:00446182 RIV/00023736:_____/15:00011089
Result on the web
<a href="http://dx.doi.org/10.1007/s11240-014-0634-x" target="_blank" >http://dx.doi.org/10.1007/s11240-014-0634-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11240-014-0634-x" target="_blank" >10.1007/s11240-014-0634-x</a>

Result language
angličtina
Original language name
Potato virus X displaying the E7 peptide derived from human papillomavirus type 16: a novel position for epitope presentation
Original language description
Potato virus X (PVX) is widely used as a peptide presentation system in plant biotechnology, mostly by fusion of desired peptides to the N-terminus of its capsid protein (CP). Considering that some epitopes can interfere with the stability and/or self-assembly of PVX CP when fused to its N-terminus, we evaluated four other possible sites for fusion using the E7 epitope derived from human papillomavirus type 16 with different tags. We prepared eight different PVX CP constructs modified with the E7 epitope fused with the 6xHis tag in both orientations (6xHis-E7, E7-6xHis), cloned them into the PVX-based vector pGR106 and expressed them transiently in Nicotiana benthamina plants. Only the fusion site located after amino acid 23 led to systemic infection of plants and the production of recombinant proteins, but no viral particles were detected. When we replaced the 6xHis with StrepII tag, the modified virus infected plants systemically, expressed proteins assembled into viral particles and
Czech name
—
Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
—

Project
<a href="/en/project/GAP501%2F12%2F1761" target="_blank" >GAP501/12/1761: Impact of different topology of heterologous antigenic determinants in PVX-based plant viral vector on their expression level and immunogenicity</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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