New positions for peptide presentation in Potato virus X capsid protein
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10296477" target="_blank" >RIV/00216208:11310/15:10296477 - isvavai.cz</a>
Alternative codes found
RIV/61389030:_____/15:00446883
Result on the web
<a href="http://dx.doi.org/10.1515/biol-2015-0019" target="_blank" >http://dx.doi.org/10.1515/biol-2015-0019</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1515/biol-2015-0019" target="_blank" >10.1515/biol-2015-0019</a>
Alternative languages
Result language
angličtina
Original language name
New positions for peptide presentation in Potato virus X capsid protein
Original language description
Potato virus X (PVX) is widely used as a peptide presentation system in plant biotechnology, mostly for transient expression of desired peptides fused to the N-terminus of its capsid protein (CP). Here we describe testing/investigation of new positions for peptide presentation based on seven putative surface loops of PVX CP. We performed bacterial expression of fourteen different PVX CPs modified by the insertion of the epitope derived from the E7 oncoprotein (E7 epitope) of Human papillomavirus type 16fused with His tag. The expression from vector pMPM-A4O in Escherichia coli MC1061 was performed to evaluate the capacity of the PVX CP platform to tolerate the insertion of E7 epitope fused with His tag in the seven putative surface loops. The immunological characteristics of expressed epitopes were assessed by Western blot using both anti-PVX CP and anti-His antibodies and by immunoelectron microscopy.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP501%2F12%2F1761" target="_blank" >GAP501/12/1761: Impact of different topology of heterologous antigenic determinants in PVX-based plant viral vector on their expression level and immunogenicity</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Open Life Sciences [online]
ISSN
2391-5412
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
1
Country of publishing house
PL - POLAND
Number of pages
9
Pages from-to
133-141
UT code for WoS article
000354077300015
EID of the result in the Scopus database
2-s2.0-84927920126